|
KMID : 0848120060310010007
|
|
International Journal of Oral Biology
2006 Volume.31 No. 1 p.7 ~ p.10
|
|
|
Identification of Enterococcus faecalis on MSE Medium Selective for Mutans Streptococci
|
|
Lee Seung-Hoon
Yoo So-Young
Kim Hwa-Sook
Kang Sook-Jin
Lim Sung-Hoon
Kim Kwang-Won
Park Jung-Min
Shin Yong-Kook
Shin Jeong-Hwan
Baek Dong-Heon
Choe Son-Jin
Kook Joong-Ki
|
|
|
Abstract
|
|
|
|
Mitis-salivarius sucrose bacitracin(MSB) medium is widely used in the selective isolation of mutans streptococci(MS), a designation for a group of oral cariogenic species. Recently, we have isolated three bacterial strains grown on MSB agar from human dental plaques. The three strains exhibited biochemical characteristics similar to those of the biotype IV of MS, with the exception that they manifested a positive reaction for arginine deaminase. The objective of this study was to identify and characterize these three clinical isolates. The bacteria were identified with biochemical tests as well as by 16S rDNA cloning and sequencing. In order to compare the antibiotics susceptibility of the clinical isolates with that of type strain, the minimum inhibitory concentrations of 9 antibiotics were determined using broth dilution assays. The results identified all of our three clinical isolates as Enterococcus faecalis. All E. faecalis strains were found to be susceptible to penicillin G, amoxicillin, augmentin, and vancomycin, but were resistant to ciprofloxacin, cefuroxim axetil, and clindamycin. Our findings indicate that E. faecalis is capable of growing on MSB agar, and suggest that the MSB medium be improved so that only MS should be recoverable on the medium, as originally devised for their selection.
|
|
|
KEYWORD
|
|
|
Enterococcus faecalis, MSB medium, 16S rDNA
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|
|